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Re: [ccp4bb] Data collection of SAD (MAD) data of Copper-containing protein
 

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CCP4bb <-- 2007 <-- March 2007 <-- 15 March 2007
Previous message:
Subject: Data collection of SAD (MAD) data of Copper-containing protein
From: Yi Xue yi-xue {- at -} NORTHWESTERN {- dot -} EDU
Date: 2007-03-15 		Next message:
Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: "Santarsiero, Bernard D {- dot -} " bds {- at -} UIC {- dot -} EDU
Date: 2007-03-15


Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: ES manian1 {- at -} VSNL {- dot -} COM
Date: 2007-03-15
Hi:

Is the resolution of the data sufficient to apply direct methods to find
only the copper atoms, then use the copper atom positions in an
old-fashioned
'heavy-atom' phasing method, combined with direct methods?

Incidentally, was the following publication of any relevance in your
efforts?
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35.
Structure determination of a 16.8 kDa copper protein at 2.1 A
resolution using anomalous scattering data with direct methods.
Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS.

ES
University of Madras

Yi Xue wrote:

>Dear all:
> We already got nice crystals of a drug-protein complex, however, MR
>failed due to the huge copies (>12) of protein molecules per asu. Protein
>itself is a small one, only ~70 aa.
> Later on, we collected MAD data of copper (copper : protein ~ 1: 1),
>Rsym of the data was around ~9%, the anomalous signals were weak, and only
>good to ~6A, the data also failed to solve the phases.
> Thus, basically, the Cu anomallous signal is very weak, and the
>crystals are kind of sensitive to radiation, it is dying but not that fast.
> I am wondering, Do we stand any chances to solve the phases by Cu MAD
>or SAD?
>
>
>Any suggestions or comments are highly appreciated?
>Yi
>
>
>

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CCP4bb <-- 2007 <-- March 2007 <-- 15 March 2007
Previous message:
Subject: Data collection of SAD (MAD) data of Copper-containing protein
From: Yi Xue yi-xue {- at -} NORTHWESTERN {- dot -} EDU
Date: 2007-03-15 		Next message:
Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: "Santarsiero, Bernard D {- dot -} " bds {- at -} UIC {- dot -} EDU
Date: 2007-03-15


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