[ccp4bb] Na acetate as purification buffer

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: twin refinement in refmac
From: Pavel Afonine pafonine {- at -} GMAIL {- dot -} COM
Date: 2012-03-02

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Subject: Re: Na acetate as purification buffer
From: Artem Evdokimov artem {- dot -} evdokimov {- at -} GMAIL {- dot -} COM
Date: 2012-03-02

Subject: Na acetate as purification buffer
From: anita p crystals {- dot -} rp {- at -} GMAIL {- dot -} COM
Date: 2012-03-02

Hi all,
Has anyone used sodium acetate buffer pH (4-5) for purifying histag protein
on Histrap column (AKTA) followed by SEC?
My protein has a pI of 9. I tried pH7.4 but it has precipitation problems.
While doing buffer screening using 24 well hanging drop I found that lower
pI onces are clear, so just thinking can I use Na acetate at pH 5 for whole
purification???

Thanks in advance
Anita

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: twin refinement in refmac
From: Pavel Afonine pafonine {- at -} GMAIL {- dot -} COM
Date: 2012-03-02

Next message:
Subject: Re: Na acetate as purification buffer
From: Artem Evdokimov artem {- dot -} evdokimov {- at -} GMAIL {- dot -} COM
Date: 2012-03-02