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Re: [ccp4bb] Data collection of SAD (MAD) data of Copper-containing protein
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: ES manian1 {- at -} VSNL {- dot -} COM
Date: 2007-03-15		Next message:
Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: Yi Xue yi-xue {- at -} NORTHWESTERN {- dot -} EDU
Date: 2007-03-15


Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: "Santarsiero, Bernard D {- dot -} " bds {- at -} UIC {- dot -} EDU
Date: 2007-03-15

It would be useful to know how you tried to solve the structure by MR.
Just because there is a large number of chains in the ASU isn't a reason
that MR will fail. At times you need to find some of the chains, do some
rebuilding, and then use that amended model for a continued search.

Bernie Santarsiero
Univ of Illinois at Chicago


On Thu, March 15, 2007 12:00 pm, Yi Xue wrote:
> Dear all:
> We already got nice crystals of a drug-protein complex, however, MR
> failed due to the huge copies (>12) of protein molecules per asu. Protein
> itself is a small one, only ~70 aa.
> Later on, we collected MAD data of copper (copper : protein ~ 1: 1),
> Rsym of the data was around ~9%, the anomalous signals were weak, and
> only
> good to ~6A, the data also failed to solve the phases.
> Thus, basically, the Cu anomallous signal is very weak, and the
> crystals are kind of sensitive to radiation, it is dying but not that
> fast.
> I am wondering, Do we stand any chances to solve the phases by Cu
> MAD
> or SAD?
>
>
> Any suggestions or comments are highly appreciated?
> Yi
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: ES manian1 {- at -} VSNL {- dot -} COM
Date: 2007-03-15		Next message:
Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: Yi Xue yi-xue {- at -} NORTHWESTERN {- dot -} EDU
Date: 2007-03-15
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