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Re: [ccp4bb] Data collection of SAD (MAD) data of Copper-containing protein |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein From: Yi Xue yi-xue {- at -} NORTHWESTERN {- dot -} EDU Date: 2007-03-15 So far, the native crystals diffracted best to 2.4A. The MAD data diffracted to 2.6~2.7A. We attempted to use phenix.hyss to identify copper atoms, and the program had hard time to identify the sites. The protein: Cu ratio is around 1:1, which is decided by ICP-AES measurement of the crystallization sample, not derived from the number of peaks in the anomalous map. The crystal contains copper as a cofactor, not a soaking derivative thing. As to oligomerization, it could be dimer or trimer, however, we don't have a model for it, since we dont know exactly, how does the drug link the monomers. I used phaser to do the MR, basically, what I did was to search one copy each time, the top 20 solutions will be used to start the search of the next copy. It stuck after finding four copies, and, I tried to change the some paramters for searching, such as percentage for peaks, it did not help. I would love the hear from experienced people about some tricks using phaser to solve some difficult MR cases. thanks a lot Yi CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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