[ccp4bb] Substitution to glycerol during crystallogenesis

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: very informative - Trends in Data Fabrication
From: Herman {- dot -} Schreuder {- at -} SANOFI {- dot -} COM Herman {- dot -} Schreuder {- at -} SANOFI {- dot -} COM
Date: 2012-04-03

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Subject: Re: very informative - Trends in Data Fabrication
From: Dyda dyda {- at -} ULTI {- dot -} NIDDK {- dot -} NIH {- dot -} GOV
Date: 2012-04-03

Subject: Substitution to glycerol during crystallogenesis
From: Toby Longwood toby {- dot -} longwood {- at -} GMAIL {- dot -} COM
Date: 2012-04-03

Dear all,

My question is related to a sample preparation.

I’m working with a complex that can be stabilized with glycerol (at least
10%) during purification. The use of detergents does not help. After
purification, the sample is homogeneous (EM) and can be concentrated
(3-4mg.mL-1) . I already set up many drops, changing several conditions
(pH, salt...) but nothing conclusive appeared.

I know that crystallogenesis in presence of glycerol works (Sousa, Acta
Cryst (1995), ...) however, because of the aspect of the drops
(precipitates that seem close to the nucleation phase), I suspect that the
glycerol can be one of the limiting factors of the protocol.

Has anybody else been already confronted to the same problem? Does someone
know if there is an alternate additive to glycerol?

Thanks in advance for suggestions/help

With best wishes

Toby

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: very informative - Trends in Data Fabrication
From: Dyda dyda {- at -} ULTI {- dot -} NIDDK {- dot -} NIH {- dot -} GOV
Date: 2012-04-03