Re: [ccp4bb] Substitution to glycerol during crystallogenesis

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From: Mark J van Raaij mjvanraaij {- at -} CNB {- dot -} CSIC {- dot -} ES
Date: 2012-04-03

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Subject: Re: Substitution to glycerol during crystallogenesis
From: Enrico Stura estura {- at -} CEA {- dot -} FR
Date: 2012-04-03

Glycerol is known to be able to reduce nucleation. This might be countered
by an increase in protein concentration.
Vera, L., Czarny, B., Georgiadis, D., Dive, V., Stura, E.A. (2011)
Practical Use of Glycerol in Protein Crystallization. Cryst. Growth & Des.
11: 2755â€“2762.

Enrico.

On Tue, 03 Apr 2012 13:49:50 +0200, Toby Longwood
wrote:

> Dear all,
>
> My question is related to a sample preparation.
>
> Iâ€™m working with a complex that can be stabilized with glycerol (at least
> 10%) during purification. The use of detergents does not help. After
> purification, the sample is homogeneous (EM) and can be concentrated
> (3-4mg.mL-1) . I already set up many drops, changing several conditions
> (pH, salt...) but nothing conclusive appeared.
>
> I know that crystallogenesis in presence of glycerol works (Sousa, Acta
> Cryst (1995), ...) however, because of the aspect of the drops
> (precipitates that seem close to the nucleation phase), I suspect that
> the
> glycerol can be one of the limiting factors of the protocol.
>
> Has anybody else been already confronted to the same problem? Does
> someone
> know if there is an alternate additive to glycerol?
>
> Thanks in advance for suggestions/help
>
> With best wishes
>
>
> Toby

--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: estura@cea.fr Fax: 33 (0)1 69 08 90 71

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: very informative - Trends in Data Fabrication
From: Mark J van Raaij mjvanraaij {- at -} CNB {- dot -} CSIC {- dot -} ES
Date: 2012-04-03

Next message:
Subject: Re: simple solution to - Trends in Data Fabrication
From: aaleshin aaleshin {- at -} BURNHAM {- dot -} ORG
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