Re: [ccp4bb] Modified N-terminal throenine?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Modified N-terminal throenine?
From: Jie Liu jie {- at -} TRIMER {- dot -} MED {- dot -} CORNELL {- dot -} EDU
Date: 2007-09-28

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Subject: ccp4 versions
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2007-09-29

Subject: Re: Modified N-terminal throenine?
From: Anthony Addlagatta anthony {- at -} UOXRAY {- dot -} UOREGON {- dot -} EDU
Date: 2007-09-28

Have you considered it to be a disordered terminus with some waters
extending the density (at least in B1 from your figures)?

Anthony
________________________________
Anthony Addlagatta, PhD
Institute of Molecular Biology
University of Oregon
Eugene, OR-97403
Phone: (541) 346-5867
Fax: (541)346-5870
Web: http://uoregon.edu/~anthony

On Sep 28, 2007, at 1:01 PM, Jie Liu wrote:

> Sure, here they are.
>
> There are three copies in ASU, all have more or less similar
> density, blue contour at 1.0sigma for 2fo-fc and magenta
> at 2.5sigma for fo-fc. The shown residues are the second
> valines which fit well.
>
> Thanks.
>
> Jie
>
> anthony@uoxray.uoregon.edu wrote:
>
>> Can you show the map?
>>
>> Anthony
>>
>>> Dear all
>>>
>>> I am refining a peptide structure with an N-terminal throenine.
>>> However, the density map of the N-terminal part looks strange,
>>> the first throenine does not fit in well while the second valine
>>> fits very well. It seems that this throenine was modified somehow
>>> during protein production. The peptide was expressed in E.Coli
>>> and cleavaged by Formic Acid and Cyanogen Bromide, then
>>> purified by HPLC and lyophilized.
>>>
>>> Does anyone have similar experience of a modified N-terminal
>>> throenine? If so, please kindly point to me a reference.
>>>
>>> Thank you.
>>>
>>> Jie Liu
>>>
>>>
>>>
>>>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Modified N-terminal throenine?
From: Jie Liu jie {- at -} TRIMER {- dot -} MED {- dot -} CORNELL {- dot -} EDU
Date: 2007-09-28

Next message:
Subject: ccp4 versions
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2007-09-29