Re: [ccp4bb] Domain swapping..... and unswapping.

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Structural Biology Post-Doctoral Position
From: "Spiller, Benjamin" benjamin {- dot -} spiller {- at -} VANDERBILT {- dot -} EDU
Date: 2007-10-05

Next message:
Subject: Re: Domain swapping..... and unswapping.
From: Lin Chen linchen {- at -} USC {- dot -} EDU
Date: 2007-10-05

Subject: Re: Domain swapping..... and unswapping.
From: Stefan Arold s {- dot -} arold {- at -} CBS {- dot -} CNRS {- dot -} FR
Date: 2007-10-05

Dear Miriam

I had that case of the 4-helix bundle FAT domain of Focal Adhesion
Kinase. It crystallised as a helix-swapped dimer (PDB ID 1k04,
1mol/ASU), as a non-swapped 4helix bundle (1ow7, 3 mol/ASU), and as a
hybrid, where 2 mols / ASU were not swapped, and one was swapped (1ow6).

With best wishes
Stefan

Miriam-Rose Ash a écrit :

>Hey all,
>
>Sorry for the off-topic post, but I'm just wondering if anyone has heard of any examples in which a single crystal contained both the domain-swapped and unswapped forms of a protein?
>
>Thanks!
>Miriam
>
>
>

--
==================================================
Stefan T. Arold, PhD
Centre de Biochimie Structurale
CNRS UMR 5048 - UM 1 - INSERM UMR 554
29 rue de Navacelles 34090
MONTPELLIER Cedex - France
email: Stefan.Arold@cbs.cnrs.fr
Phone: +33 (0)4.67.41.77.02
Fax: +33 (0)4.67.41.79.13
==================================================

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Structural Biology Post-Doctoral Position
From: "Spiller, Benjamin" benjamin {- dot -} spiller {- at -} VANDERBILT {- dot -} EDU
Date: 2007-10-05

Next message:
Subject: Re: Domain swapping..... and unswapping.
From: Lin Chen linchen {- at -} USC {- dot -} EDU
Date: 2007-10-05