Re: [ccp4bb] off topic detection of oligomerisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: off topic detection of oligomerisation
From: Carlos Kikuti kikuti {- at -} GMAIL {- dot -} COM
Date: 2012-07-11

Urea?!?!? My arm hair went up.

Anyways, DLS (Dynamic Light Scattering) might help. Hard to imagine something quicker and easier, provided that someone next to you already has a DLS machine.

(sorry for the late answer)

Carlos

Em 26/06/2012, às 15:10, Brad Bennett escreveu:

> Native PAGE (i.e. BN-PAGE), light scattering (i.e. MALLS)
>
> Not quick and easy but could work: AUC (i.e. a sedimentation equilibrium experiment)
>
> -Brad
>
> On Tue, Jun 26, 2012 at 9:01 AM, Careina Edgooms wrote:
> Dear ccp4 bulletin board
>
> I apologise for off topic question. I wonder if anybody knows of a good method to detect oligomerisation?
> I suspect an equilibrium intermediate is forming oligomers based on tryptophan fluorescence showing an exposed tryptophan becoming buried in a hydrophobic region. I would like to perform some experiments to prove this. Cross linking is not working for me and neither is SEC HPLC due to technical issues with the urea I think.
> Are there any other quick and easy techniques that any of you can think of off the cuff? Shifts in equilibrium unfolding curves done at different concentrations will probably show something because of the law of mass action but this is a big job needing many replicates so I wonder if there are any other simple methods available?
>
> Thanks
> Careina
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Mercury phenylglyoxal
From: David Briggs drdavidcbriggs {- at -} GMAIL {- dot -} COM
Date: 2012-07-11

Next message:
Subject: Re: FE-Sulfur proteins
From: "Edward A {- dot -} Berry" BerryE {- at -} UPSTATE {- dot -} EDU
Date: 2012-07-11