Re: [ccp4bb] FE-Sulfur proteins

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: off topic detection of oligomerisation
From: Carlos Kikuti kikuti {- at -} GMAIL {- dot -} COM
Date: 2012-07-11

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Subject: Re: FE-Sulfur proteins
From: "Edward A {- dot -} Berry" BerryE {- at -} UPSTATE {- dot -} EDU
Date: 2012-07-11

Good to know about this ISC operon!

Do you know if it is specific for Rieske-type His2/Cys2 Fe2S2 clusters,
or for FeS clusters in general?
Thus wild-type E. coli is already making three types of ISC clusters
for succinate dehydrogenase or fumarate reductase (although some help
might be needed for overexpression) but I don't know if it has
any Rieske-type clusters.

Kelly Daughtry wrote:
> Jan,
> Do you express the protein with the /E. coli isc/ iron-sulfur cluster synthetic operon?
> I found great success, see:
>
> Daughtry, KD et. al. JACS 2012
> http://pubs.acs.org/doi/full/10.1021/ja2111898
>
> - Kelly Daughtry
>
> *******************************************************
> Kelly Daughtry, Ph.D.
> Post-Doctoral Fellow, Raetz Lab
> Biochemistry Department
> Duke University
> Alex H. Sands, Jr. Building
> 303 Research Drive
> RM 250
> Durham, NC 27710
> P: 919-684-5178
> *******************************************************
>
>
> On Wed, Jul 11, 2012 at 6:22 AM, Jan Rashid Umar
> > wrote:
>
> Dear All,
>
> I will be grateful to your suggestions about Iron-Sulfur cluster protein purification.
> My colleague has some problems with the purification and it seems that the iron-sulfur
> cluster might be deformed, and protein is aggregated. The purification is done under
> aerobic (normal condition), and is there some method to reintegrate iron sulfur
> cluster back into the protein molecule under these conditions. Can anybody suggest
> some literature, protocol or something that can improve the protein aggregation?
>
> I look forward to hearing from you.
>
> Best wishes,
>
> Jan
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: off topic detection of oligomerisation
From: Carlos Kikuti kikuti {- at -} GMAIL {- dot -} COM
Date: 2012-07-11

Next message:
Subject: Re: Mercury phenylglyoxal
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2012-07-11