Re: [ccp4bb] One little clash

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: One little clash
From: "Lukacs, Christine" christine {- dot -} lukacs {- at -} ROCHE {- dot -} COM
Date: 2012-07-11

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Subject: Re: One little clash
From: James Stroud xtald00d {- at -} GMAIL {- dot -} COM
Date: 2012-07-11

It looks like dityrosine, usually caused by radiation damage (I think UV is the usual culprit).

http://www.nugowiki.org/images/thumb/c/ca/HMDB06045.png/220px-HMDB06045.png

James

On Jul 11, 2012, at 1:37 PM, Lukacs, Christine wrote:

> Hi all-
>
> I have a protein that crystallizes in I422, and diffracts well, between 1.3-1.7A. Beautiful density, slightly higher final R-factors than you might expect at this resolution (low to mid 20s). The density is all beautiful, except that I have this one little clash, between a few atoms from a tyrosine and its symmetry mate. In this picture I have it modeled as an Alanine and you can see the two tyrosine rings interlocking; and there is clearly no alternate conformation.
>
>
>
> Since it is not near my site of interest, I have been pretty much ignoring it, going through refinement with it as an alanine, then changing it at the very end to a tyrosine and just minimizing B-s, no positional. Now that I plan to publish a bunch of these, I should probably figure out what is really going on. Any insights?
>
> Thanks
> Christine
> Christine Lukacs
> Roche
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: One little clash
From: "Lukacs, Christine" christine {- dot -} lukacs {- at -} ROCHE {- dot -} COM
Date: 2012-07-11

Next message:
Subject: Re: One little clash
From: James Stroud xtald00d {- at -} GMAIL {- dot -} COM
Date: 2012-07-11