Re: [ccp4bb] off topic Thermal shift assay

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: off topic Thermal shift assay
From: anita p crystals {- dot -} rp {- at -} GMAIL {- dot -} COM
Date: 2012-07-19

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From: Boaz Shaanan bshaanan {- at -} EXCHANGE {- dot -} BGU {- dot -} AC {- dot -} IL
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Subject: Re: off topic Thermal shift assay
From: Hüsnü_Topal huesnue {- dot -} topal {- at -} RUB {- dot -} DE
Date: 2012-07-19

Dear Anita,

here is a link to a talk with recommended literature:
http://thermofluor.org/resources/Maksel_Protein-Stability.pdf.
Probably, you will find there the answers you need.

I recommend to try several dyes if available, due to the different
behaviour of proteins and no general rule for doing thermal shift assays.

good luck

Hüsnü Topal, Dr. rer. nat.

Protein Crystallography & Bioinformatics
Workgroup of Prof. Dr. W. Welte
Universitätsstr. 10
78457 Konstanz

Am 19.07.2012 10:26, schrieb anita p:
> Hi All,
> I want to use a thermofluor for the thermal shift assay. My proteins
> are cytoplasmic truncations of membrane protein. I have read about
> ANS, sypro-orange and CPM. Which is the once that is popularly used by
> the crystallographers for condition optimization for crystallization ??
>
> I have read that it sypro orange is not good for hydrophobic proteins
> and CPM can't be used with DTT or bME in the buffer.
> I am a bit confused .
> Please help
> thanks in advance
> Anita

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: off topic Thermal shift assay
From: anita p crystals {- dot -} rp {- at -} GMAIL {- dot -} COM
Date: 2012-07-19

Next message:
Subject: Re: peptide fragment
From: Boaz Shaanan bshaanan {- at -} EXCHANGE {- dot -} BGU {- dot -} AC {- dot -} IL
Date: 2012-07-19