Re: [ccp4bb] Nucleophilic attack by the side-chain carboxyl group of Asp?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Another lame question
From: bhaba krishna Das bhabakd {- at -} GMAIL {- dot -} COM
Date: 2012-07-21

Next message:
Subject: Re: off topic Thermal shift assay
From: Artem Evdokimov artem {- dot -} evdokimov {- at -} GMAIL {- dot -} COM
Date: 2012-07-21

Subject: Re: Nucleophilic attack by the side-chain carboxyl group of Asp?
From: Peter Hsu hsuuper {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2012-07-21

I too am also studying the reaction mechanism of an enzyme, but my chemistry/enzymatic biochemistry is rather weak after many years of non-use and no review. Does anyone know just as a general rule which residues are the best to worst nucleophiles?

Sorry if this seems rather presumptuous, just not sure where to look in literature for a summary of these things.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Another lame question
From: bhaba krishna Das bhabakd {- at -} GMAIL {- dot -} COM
Date: 2012-07-21

Next message:
Subject: Re: off topic Thermal shift assay
From: Artem Evdokimov artem {- dot -} evdokimov {- at -} GMAIL {- dot -} COM
Date: 2012-07-21