Re: [ccp4bb] best strategy to establish stable expression in mammalian cells

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: best strategy to establish stable expression in mammalian cells
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2012-08-17

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Subject: Evaluating crystallogarphic experiment
From: Yuri Pompeu yuri {- dot -} pompeu {- at -} UFL {- dot -} EDU
Date: 2012-08-17

Subject: Re: best strategy to establish stable expression in mammalian cells
From: John K Lee kwlee {- at -} MSG {- dot -} UCSF {- dot -} EDU
Date: 2012-08-17

Hi Jerry,

Perhaps this Nature protocols paper from the Stroud Lab at UCSF might be useful.

Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells

http://www.nature.com/nprot/journal/v7/n3/full/nprot.2011.453.html

-john

On Aug 17, 2012, at 3:38 PM, Jerry McCully wrote:

> Dear All;
>
> We are planning to establish stable CHO or 293 cell clones to express some receptors.
>
> I heard that it is time consuming to select high expression clones using pcDNA3.1 vectors.
>
> As our crystallographer are dealing with all kinds of proteins, can anyone recommend a best strategy for mammalian cell expression?
>
> Thank you so much and have a nice weekend,
>
> Jerry McCully
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: best strategy to establish stable expression in mammalian cells
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2012-08-17

Next message:
Subject: Evaluating crystallogarphic experiment
From: Yuri Pompeu yuri {- dot -} pompeu {- at -} UFL {- dot -} EDU
Date: 2012-08-17