Re: [ccp4bb] Evaluating crystallogarphic experiment

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Evaluating crystallogarphic experiment
From: Yuri Pompeu yuri {- dot -} pompeu {- at -} UFL {- dot -} EDU
Date: 2012-08-17

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Subject: mirror symmetry
From: "Frances C {- dot -} Bernstein" fcb {- at -} BERNSTEIN-PLUS-SONS {- dot -} COM
Date: 2012-08-19

Subject: Re: Evaluating crystallogarphic experiment
From: Roger Rowlett rrowlett {- at -} COLGATE {- dot -} EDU
Date: 2012-08-18

Quite common. And it may be a good sign for crystallizability in those
conditions. As the concentration gradients dissipate from the initially
mixed drop, your protein re-solubilizes.

Roger Rowlett
On Aug 17, 2012 5:31 PM, "Yuri Pompeu" wrote:

> Hello everyone,
> I have just made a curious observation.
> I purified an enzyme domain and concentrated it to around 16 mg/mL without
> much mischief.
> I then proceeded to set-up some drops. After 5 min most of the drops had a
> muddy appearance to them which led me to think I was probably too
> concentrated in either protein and/or precipitant.
> Curiously I went back to look at the drops after an additional 30 min and
> they all look pretty clear with no appreciable precipitation.
> Has anyone encountered this situation or a similar one before?
> Any input/shared experience is welcome.
> Best,
> Yuri
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Evaluating crystallogarphic experiment
From: Yuri Pompeu yuri {- dot -} pompeu {- at -} UFL {- dot -} EDU
Date: 2012-08-17

Next message:
Subject: mirror symmetry
From: "Frances C {- dot -} Bernstein" fcb {- at -} BERNSTEIN-PLUS-SONS {- dot -} COM
Date: 2012-08-19