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[ccp4bb] Is phophorylation possible in E. coli expression system?
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Cost-effective imaging systems: recommendations and opinions requested
From: "Page, Rebecca" Rebecca_Page {- at -} BROWN {- dot -} EDU
Date: 2007-10-26		Next message:
Subject: Re: Cost-effective imaging systems: recommendations and opinions requested
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-10-26


Subject: Is phophorylation possible in E. coli expression system?
From: Narayanan Ramasubbu ramasun1 {- at -} UMDNJ {- dot -} EDU
Date: 2007-10-26

Dear All:
This is not crystallography related and does not belong to this group,
but I would like to pose this to all who are working with proteins
expressed in an E. coli BL21 (DE3) or Rosetta. Is it possible for a
protein to be phosphorylated during expression? At least my
understanding was that post-translational modifications are not
possible in E. coli. However, recently we expressed a protein and
noticing that there are lot of potential tyrosine phosphorylation
sites, we checked the expressed protein on a gel using a stain that
detect phosphates. (Please do not say Are you crazy? Why would you
check.. etc). Lo and behold, there lights up the band. Hence my
question to you all. I could not google or mine from PubMed specific
references that exist regarding this. Please enlighten me.
Thanks a lot.
Also, I would like to take this opportunity to thank everybody for all
the help whenever ask some off-topic question like ths.
Subbu

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Cost-effective imaging systems: recommendations and opinions requested
From: "Page, Rebecca" Rebecca_Page {- at -} BROWN {- dot -} EDU
Date: 2007-10-26		Next message:
Subject: Re: Cost-effective imaging systems: recommendations and opinions requested
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-10-26
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