Re: [ccp4bb] the B facot of soaked substrate

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: the B facot of soaked substrate
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2007-11-01

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Subject: Re: the B facot of soaked substrate
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2007-11-01

Subject: Re: the B facot of soaked substrate
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2007-11-01

Dear Herman:
It is due to the occupancy obviously.
Previously, someone mensioned that in this resolution it is not suitable to
refine the occupancy.
So I think it is better for me to keep the occupancy to 1.
Do you have some case for this situation in the PDB bank?

Thanks.

On 11/1/07, Herman.Schreuder@sanofi-aventis.com <
Herman.Schreuder@sanofi-aventis.com> wrote:
>
> Deart Jamu,
> You probably do not have full occupancy. Set the occupancy of the
> inhibitor to say 0.6 and refine again and see what happens to the
> B-factors.
> Herman
>
> **
> ------------------------------
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *Jiamu
> Du
> *Sent:* Thursday, November 01, 2007 1:05 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] the B facot of soaked substrate
>
>
> Dear All:
> I am refining a protein structure with a soaked substrate.
> The resolution is 2.5 A. The B factor of protein is around 40, while the B
> factor of the soaked substrate is as high as 80.
> The density looks fine.
> Is this structure acceptable? Or is there anyone who can give me an
> example of this situation in the PDB bank?
> Thanks.
>
>
> --
> Jiamu Du
> State Key Laboratory of Molecular Biology
> Institute of Biochemistry and Cell Biology Shanghai Institutes for
> Biological Sciences
> Chinese Academy of Sciences (CAS)
>

--
Jiamu Du
State Key Laboratory of Molecular Biology
Institute of Biochemistry and Cell Biology Shanghai Institutes for
Biological Sciences
Chinese Academy of Sciences (CAS)

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: the B facot of soaked substrate
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2007-11-01

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Subject: Re: the B facot of soaked substrate
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2007-11-01