[ccp4bb] Ni-NTA purifications contaminated with E coli Glucosamine-fructose-6-phosphate aminotransferase

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: opinion : phaser RMSD vis-a-vis LLG, Z-score
From: "Bryan W {- dot -} Lepore" bryanlepore {- at -} MAIL {- dot -} UTEXAS {- dot -} EDU
Date: 2007-11-02

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Subject: Re: Ni-NTA purifications contaminated with E coli Glucosamine-fructose-6-phosphate aminotransferase
From: Christian Biertumpfel biertumpfelc {- at -} NIDDK {- dot -} NIH {- dot -} GOV
Date: 2007-11-02

Subject: Ni-NTA purifications contaminated with E coli Glucosamine-fructose-6-phosphate aminotransferase
From: Ailong Ke ak425 {- at -} CORNELL {- dot -} EDU
Date: 2007-11-02

>Hello,

We are trying to purify an N-terminal His6-tagged protein from E.
coli, and the prep was contaminated with the E coli
Glucosamine-fructose-6-phosphate aminotransferase, which co-purifies
with my protein of interest in subsequent ion-exchange and sizing
columns. This protein appears to be a common contamination in the
Ni-NTA purifications. Does anyone have tricks to get rid of it?
Thanks.

Ailong

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: opinion : phaser RMSD vis-a-vis LLG, Z-score
From: "Bryan W {- dot -} Lepore" bryanlepore {- at -} MAIL {- dot -} UTEXAS {- dot -} EDU
Date: 2007-11-02