[ccp4bb] Dissolving s-adenosylhomocysteine

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CCP4bb <-- 2007 <-- November 2007 <-- 06 November 2007

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Subject: [Protein-crystallography] November 2007 issue of the IUCr Computing Commission Newsletter on-line (fwd)
From: Gerard DVD Kleywegt gerard {- at -} XRAY {- dot -} BMC {- dot -} UU {- dot -} SE
Date: 2007-11-06

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Subject: Re: Dissolving s-adenosylhomocysteine
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2007-11-06

Subject: Dissolving s-adenosylhomocysteine
From: Ray Changrui Lu changrui {- at -} GMAIL {- dot -} COM
Date: 2007-11-06

Hi all,

I am in the process of obtaining crystals in complex with
s-adenosylhomocysteine but this compound doesn't dissolve in normal
pH. The final concentration needs to be in mM range so the stock
ideally needs to be at least 50mM. I know SAH is quite soluble in DMSO
but is there anyway to get it in aqueous solution without organic
solvent or strong acid? Thank you very much in advance.

Ray

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CCP4bb <-- 2007 <-- November 2007 <-- 06 November 2007

Next message:
Subject: Re: Dissolving s-adenosylhomocysteine
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2007-11-06