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Re: [ccp4bb] Dissolving s-adenosylhomocysteine |
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CCP4bb navigationCCP4bb <-- 2007 <-- November 2007 <-- 06 November 2007Subject: Re: Dissolving s-adenosylhomocysteine From: Ralf JAUCH jauchr {- at -} GIS {- dot -} A-STAR {- dot -} EDU {- dot -} SG Date: 2007-11-06 I've got a similar concern and contacted SIGMA for advice. I got the following response: "We test routinely solubility in water (100 mg/ml). A clear to slightly hazy light yellow solution is yielded. This material is 80-90% pure when prepared, but is very unstable. As much as 10% purity loss per day at 25 °C has been noted. Further it reacts with water. Solution Stability according to Data for Biochemical Research, 3rd ed., p. 2 (1987): S-adenosylmethionine is relatively stable in acid solutions and very unstable under alkaline conditions. Stock solutions should be stable when stored at -20° C at pH 2.5-4.0 for one month.The solutions should be warmed up just before use. In addition, solutions after 24 hours at 30°C decomposed 10% at pH 6.6, 32% at pH 7.8 and 70% at pH 8.8." The above is for SAM I'm still waiting for a response regarding SAH. Ralf -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Ray Changrui Lu Sent: Wednesday, November 07, 2007 2:48 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Dissolving s-adenosylhomocysteine Hi all, I am in the process of obtaining crystals in complex with s-adenosylhomocysteine but this compound doesn't dissolve in normal pH. The final concentration needs to be in mM range so the stock ideally needs to be at least 50mM. I know SAH is quite soluble in DMSO but is there anyway to get it in aqueous solution without organic solvent or strong acid? Thank you very much in advance. Ray CCP4bb navigationCCP4bb <-- 2007 <-- November 2007 <-- 06 November 2007 |
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