[ccp4bb] Pointless and space group P 4 3 2

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Poinless and space group P 4 3 2
From: Kristof Van Hecke kristof {- dot -} vanhecke {- at -} CHEM {- dot -} KULEUVEN {- dot -} BE
Date: 2007-11-13

Next message:
Subject: Re: Poinless and space group P 4 3 2
From: "Manfred S {- dot -} Weiss" msweiss {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2007-11-13

Subject: Pointless and space group P 4 3 2
From: Kristof Van Hecke kristof {- dot -} vanhecke {- at -} CHEM {- dot -} KULEUVEN {- dot -} BE
Date: 2007-11-13

Dear all,

When running Pointless 1.2.0 (see summary below), I get the cubic
space group P 4 3 2
(mosflm gave P 2 3 or P 4 3 2)

Best Solution space group P 4 3 2
Reindex operator: [h,k,l]
Laue group probability: 1.000
Systematic absence probability: 0.975
Total probability: 0.975
Space group confidence: 0.964
Laue group confidence 1.000

However, although I have multiple models (ensemble) with
approximately 30% sequence identity (44% homology), I'm not able to
get any reasonable solution with Phaser.
As we know the protein can form dimers and even trimers, is it
possible that Poinless is giving a higher space group because of
pseudo-symmetry in this case..?

Does anyone has got experience with Pointless and this kind of space
group please..?

Many thanks

Kristof

--------------------------------------
Kristof Van Hecke, PhD
Biomoleculaire Architectuur
Celestijnenlaan 200 F
B-3001 Heverlee (Leuven)
Tel: +32(0)16327477
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Poinless and space group P 4 3 2
From: Kristof Van Hecke kristof {- dot -} vanhecke {- at -} CHEM {- dot -} KULEUVEN {- dot -} BE
Date: 2007-11-13

Next message:
Subject: Re: Poinless and space group P 4 3 2
From: "Manfred S {- dot -} Weiss" msweiss {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2007-11-13