Re: [ccp4bb] Poinless and space group P 4 3 2

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Poinless and space group P 4 3 2
From: "Manfred S {- dot -} Weiss" msweiss {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2007-11-13

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Subject: Re: Pointless and space group P 4 3 2
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-11-13

Subject: Re: Poinless and space group P 4 3 2
From: Phil Evans pre {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-11-13

You should certainly try the other P 4x 3 2 space groups (x=1,2,3) in
case the assignment of screw axes is wrong
Phil

On 13 Nov 2007, at 13:17, Kristof Van Hecke wrote:

> Dear all,
>
> When running Pointless 1.2.0 (see summary below), I get the cubic
> space group P 4 3 2
> (mosflm gave P 2 3 or P 4 3 2)
>
>
>
> Best Solution space group P 4 3 2
> Reindex operator: [h,k,l]
> Laue group probability: 1.000
> Systematic absence probability: 0.975
> Total probability: 0.975
> Space group confidence: 0.964
> Laue group confidence 1.000
>
>
>
> However, although I have multiple models (ensemble) with
> approximately 30% sequence identity (44% homology), I'm not able to
> get any reasonable solution with Phaser.
> As we know the protein can form dimers and even trimers, is it
> possible that Poinless is giving a higher space group because of
> pseudo-symmetry in this case..?
>
> Does anyone has got experience with Pointless and this kind of
> space group please..?
>
> Many thanks
>
> Kristof
>
>
> --------------------------------------
> Kristof Van Hecke, PhD
> Biomoleculaire Architectuur
> Celestijnenlaan 200 F
> B-3001 Heverlee (Leuven)
> Tel: +32(0)16327477
> --------------------------------------
>
>
>
>
>
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Poinless and space group P 4 3 2
From: "Manfred S {- dot -} Weiss" msweiss {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2007-11-13

Next message:
Subject: Re: Pointless and space group P 4 3 2
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-11-13