Re: [ccp4bb] B-factors, H/D exchange and protein flexibility

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: B-factors, H/D exchange and protein flexibility
From: Ethan Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-11-13

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Subject: Postdoc and PhD position
From: Klaus Piontek klaus {- dot -} piontek {- at -} OCBC {- dot -} UNI-FREIBURG {- dot -} DE
Date: 2007-11-13

Subject: Re: B-factors, H/D exchange and protein flexibility
From: Jayashankar s {- dot -} jayashankar {- at -} GMAIL {- dot -} COM
Date: 2007-11-13

Dear Brad,

the flexibility, that is how far a protein is stabilized with cold and heat
denaturation of a protein,

this inturn depends on

1.solvation enthalpy of nonpolar moieties(need more energy,so there needs to
be some randomness)
2.shift in temparature due to the enthalpy associated with transfer of
peptide group into water
3.its all depend truly on enthalpy convergence temperature and entropy
convergence temperature.

infact both quantities are characterised with large enthapy and entropy
compensation and; the temperature.

thanks

On Nov 13, 2007 6:07 PM, Ethan Merritt wrote:

> On Tuesday 13 November 2007 06:41, Brad Bennett wrote:
> >
> > I would be interested then to know how the community feels about the
> > correlation of B-factors to protein flexibility. It is generally
> accepted
> > that these are linked but are there any new papers that address this?
>
> This is the basis of TLSMD analysis, and TLS refinement in general.
> TLSM is described in
> Painter & Merritt (2006) Acta Cryst. D62, 439-450
>
> >
>
>
> --
> Ethan A Merritt Courier Deliveries: 1959 NE Pacific
> Dept of Biochemistry
> Health Sciences Building
> University of Washington - Seattle WA 98195-7742
>

--
S.Jayashankar
Research Student
Institute for Biophysical Chemistry
Hannover Medical School
Germany

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: B-factors, H/D exchange and protein flexibility
From: Ethan Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-11-13

Next message:
Subject: Postdoc and PhD position
From: Klaus Piontek klaus {- dot -} piontek {- at -} OCBC {- dot -} UNI-FREIBURG {- dot -} DE
Date: 2007-11-13