[ccp4bb] DTT sensitive?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Marius Schmidt marius {- dot -} schmidt {- at -} PH {- dot -} TUM {- dot -} DE
Date: 2007-11-13

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From: harry powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-11-13

Subject: DTT sensitive?
From: Joe zzjsail {- at -} GMAIL {- dot -} COM
Date: 2007-11-13

Hi there,
I see enormous precipitate of my receptor protein when I take it out
of freezer. But all the precipitate dissolved quickly after I added
1mM DTT to the solution. Does this mean that some surface Cys are
causing problem? Or why is the protein so sensitive to DTT?
Anybody experienced this kind?
Any advice is appreciated.
-Joe

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject:
From: Marius Schmidt marius {- dot -} schmidt {- at -} PH {- dot -} TUM {- dot -} DE
Date: 2007-11-13

Next message:
Subject:
From: harry powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-11-13