Re: [ccp4bb] DTT sensitive?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: DTT sensitive?
From: "Sanishvili, Ruslan" rsanishvili {- at -} ANL {- dot -} GOV
Date: 2007-11-13

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Subject: Re: DTT sensitive?
From: Joe zzjsail {- at -} GMAIL {- dot -} COM
Date: 2007-11-13

Subject: Re: DTT sensitive?
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2007-11-13

Did you check the pH of your DTT and the protein solution before and after?
I have had some experience with DTT changing pH (as might well be
expected...).

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.467.4049
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

----- Original Message -----
From: "Joe"
To:
Sent: Tuesday, November 13, 2007 2:30 PM
Subject: [ccp4bb] DTT sensitive?

> Hi there,
> I see enormous precipitate of my receptor protein when I take it out
> of freezer. But all the precipitate dissolved quickly after I added
> 1mM DTT to the solution. Does this mean that some surface Cys are
> causing problem? Or why is the protein so sensitive to DTT?
> Anybody experienced this kind?
> Any advice is appreciated.
> -Joe
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: DTT sensitive?
From: "Sanishvili, Ruslan" rsanishvili {- at -} ANL {- dot -} GOV
Date: 2007-11-13

Next message:
Subject: Re: DTT sensitive?
From: Joe zzjsail {- at -} GMAIL {- dot -} COM
Date: 2007-11-13