Re: [ccp4bb] DTT sensitive?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: DTT sensitive?
From: Joe zzjsail {- at -} GMAIL {- dot -} COM
Date: 2007-11-13

Next message:
Subject: Re: DTT sensitive?
From: Joe zzjsail {- at -} GMAIL {- dot -} COM
Date: 2007-11-13

Subject: Re: DTT sensitive?
From: Joe zzjsail {- at -} GMAIL {- dot -} COM
Date: 2007-11-13

The precipitate does not disappear automaticly if I don't add DTT.
If it's the precipitate of imidazole, then why DTT can dissolve it?
thanks

On 11/13/07, Sanishvili, Ruslan wrote:
> This may not apply in your case but it is not uncommon for a protein to
> "precipitate" in a microcrystalline shower when put in cold. Once it
> worms up, the crystals dissolve and the precipitate clears up. It is
> easy to check under a high magnification microscope.
> Cheers,
> N.
>
>
> Ruslan Sanishvili (Nukri), Ph.D.
>
> GM/CA-CAT, Bld. 436, D007
> Biosciences Division, ANL
> 9700 S. Cass Ave.
> Argonne, IL 60439
>
> Tel: (630)252-0665
> Fax: (630)252-0667
> rsanishvili@anl.gov
>
>
>
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Joe
> Sent: Tuesday, November 13, 2007 2:30 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] DTT sensitive?
>
> Hi there,
> I see enormous precipitate of my receptor protein when I take it out of
> freezer. But all the precipitate dissolved quickly after I added 1mM DTT
> to the solution. Does this mean that some surface Cys are causing
> problem? Or why is the protein so sensitive to DTT?
> Anybody experienced this kind?
> Any advice is appreciated.
> -Joe
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: DTT sensitive?
From: Joe zzjsail {- at -} GMAIL {- dot -} COM
Date: 2007-11-13

Next message:
Subject: Re: DTT sensitive?
From: Joe zzjsail {- at -} GMAIL {- dot -} COM
Date: 2007-11-13