Re: [ccp4bb] About deblock N-terminus

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Malonate Crystals?
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2007-11-16

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Subject: Re: Malonate Crystals?
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-11-16

Subject: Re: About deblock N-terminus
From: verdino {- at -} SCRIPPS {- dot -} EDU verdino {- at -} SCRIPPS {- dot -} EDU
Date: 2007-11-16

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Hi,

could as well be that your first residue is Gln which frequently cylizes
to pyroglutamate which resists Edman chemistry. In that case you can
deblock your N-terminus with pyroglutamate aminopeptidase (you can get it
from Takara, Sigma, Boehringer, Pierce - whicheverone you like ;-)). THere
are well working standard protocols.

Cheers, Petra

--
PLEASE NOTE THE NEW PHONE NUMBER!!!

Petra Verdino, PhD
Research Associate
The Scripps Research Institute, BCC206
10550 North Torrey Pines Road
CA 92037 La Jolla, USA
tel:1-858-784-2945
fax:1-858-784-2980

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Malonate Crystals?
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2007-11-16

Next message:
Subject: Re: Malonate Crystals?
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-11-16