Re: [ccp4bb] Protein-detergent micelle sizes

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Protein-detergent micelle sizes
From: Savvas Savvides savvas {- dot -} savvides {- at -} UGENT {- dot -} BE
Date: 2007-11-20

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Subject: Re: Unmerged output from Scala
From: "Winter, G (Graeme)" g {- dot -} winter {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-11-20

Subject: Re: Protein-detergent micelle sizes
From: Edward Berry eaberry {- at -} LBL {- dot -} GOV
Date: 2007-11-20

In case you end up compiling your own list, here is one entry:

von Jagow and co-workers (Biochim Biophys Acta. 1977 462(3):549-58.)
used tritiated Triton X-100 to measure the binding to Complex III

"5. In accordance with the high polarity the amount of bound
detergent is relatively low, it amounts to 0.2 g Triton X-100/g
protein. The amount of Triton bound to 1 mol of b-c1-dimer corresponds
to the molecular weight of the Triton micelle."

The bc1 dimer MW is ~480 kDa, so .2 g/g -> 96 kDa detergent

Jacob Keller wrote:
> Dear Crystallographers,
>
> I am trying to gather literature values on the size of the
> detergent/micelle belts on solublized membrane proteins. Does anybody
> know of a good repository of this info, whether as a database or as a
> review paper, or otherwise? The rough values of 15-25kD to 35-50kD were
> given in one recent BB posting, but I was looking for a more systematic
> tabulation...
>
> Thanks,
>
> Jacob Keller
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.467.4049
> cel: 773.608.9185
> email: j-keller2@northwestern.edu
> *******************************************

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Protein-detergent micelle sizes
From: Savvas Savvides savvas {- dot -} savvides {- at -} UGENT {- dot -} BE
Date: 2007-11-20

Next message:
Subject: Re: Unmerged output from Scala
From: "Winter, G (Graeme)" g {- dot -} winter {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-11-20