Re: [ccp4bb] Protein-detergent micelle sizes

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: ISGO 2008 First Announcement
From: Ian Berry ian {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
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Subject: Re: Protein-detergent micelle sizes
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2007-11-20

Subject: Re: Protein-detergent micelle sizes
From: "R {- dot -} M {- dot -} Garavito" garavito {- at -} MSU {- dot -} EDU
Date: 2007-11-20

Jacob,

Savvas' suggestion about the the Calbiochem and Anatrace product
catalogs is a good one, particularly as the latter is fairly up to
date regarding the newest detergents. Reviews tend to be out of date
quickly.

Also, concerning methods and the odd useful measurements of detergent-
binding to membrane proteins, get almost any paper written by Marc le
Maire on the subject. Finally, since everyone now has "access" to
analytical ultracentrifugation, consider doing AUC with density
matching with D2O, sucrose, or Nycodenz. Ariel Lustig's paper on the
subject (Biochimica et Biophysica Acta 1464, 199-206, 2000) is an
excellent guide to the method, although it took nearly 20 years to
get him to publish his Model E data. A lot of his useful measurements
were put in diplom and Ph.D. theses at the Biozentrum and virtually
forgotten.

Cheers,

Michael

****************************************************************
R. Michael Garavito, Ph.D.
Professor of Biochemistry & Molecular Biology
513 Biochemistry Bldg.
Michigan State University
East Lansing, MI 48824-1319
Office: (517) 355-9724 Lab: (517) 353-9125
FAX: (517) 353-9334 Email: garavito@msu.edu
****************************************************************

On Nov 20, 2007, at 4:22 AM, Edward Berry wrote:

> In case you end up compiling your own list, here is one entry:
>
> von Jagow and co-workers (Biochim Biophys Acta. 1977 462(3):549-58.)
> used tritiated Triton X-100 to measure the binding to Complex III
>
> "5. In accordance with the high polarity the amount of bound
> detergent is relatively low, it amounts to 0.2 g Triton X-100/g
> protein. The amount of Triton bound to 1 mol of b-c1-dimer
> corresponds
> to the molecular weight of the Triton micelle."
>
> The bc1 dimer MW is ~480 kDa, so .2 g/g -> 96 kDa detergent
>
> Jacob Keller wrote:
>> Dear Crystallographers,
>> I am trying to gather literature values on the size of the
>> detergent/micelle belts on solublized membrane proteins. Does
>> anybody know of a good repository of this info, whether as a
>> database or as a review paper, or otherwise? The rough values of
>> 15-25kD to 35-50kD were given in one recent BB posting, but I was
>> looking for a more systematic tabulation...
>> Thanks,
>> Jacob Keller
>> *******************************************
>> Jacob Pearson Keller
>> Northwestern University
>> Medical Scientist Training Program
>> Dallos Laboratory
>> F. Searle 1-240
>> 2240 Campus Drive
>> Evanston IL 60208
>> lab: 847.467.4049
>> cel: 773.608.9185
>> email: j-keller2@northwestern.edu
>> *******************************************
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: ISGO 2008 First Announcement
From: Ian Berry ian {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2007-11-20

Next message:
Subject: Re: Protein-detergent micelle sizes
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2007-11-20