Re: [ccp4bb] Protein-detergent micelle sizes

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Protein-detergent micelle sizes
From: "R {- dot -} M {- dot -} Garavito" garavito {- at -} MSU {- dot -} EDU
Date: 2007-11-20

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From: Andrew Torelli andrew_torelli {- at -} URMC {- dot -} ROCHESTER {- dot -} EDU
Date: 2007-11-20

Subject: Re: Protein-detergent micelle sizes
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2007-11-20

Jacob,

Whether the calbiochem/anatrace catalogues will work for you depends on what you want to know. They do have useful info on micelle sizes (aggregation #s, cmc's etc), but these are values for micelles alone, either in water or 0.1 M salt or something like that. If your question is how much detergent is actually associated with a particular protein you'll unfortunately have to measure that yourself (see Michaels message), since this will be protein and condition dependent (ie a large membrane protein will have more detergent associated with it than a small one). There was a thread on this subject on this board a couple weeks back i think.

Cheers, Bert

Bert van den Berg
University of Massachusetts Medical School
Program in Molecular Medicine
Biotech II, 373 Plantation Street, Suite 115
Worcester MA 01605
Phone: 508 856 1201 (office); 508 856 1211 (lab)
e-mail: bert.vandenberg@umassmed.edu
http://www.umassmed.edu/pmm/faculty/vandenberg.cfm

________________________________

From: CCP4 bulletin board on behalf of Savvas Savvides
Sent: Tue 11/20/2007 3:53 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Protein-detergent micelle sizes

Hi Jacob,
the Calbiochem and Anatrace product catalogues will do the trick for
you.
best wishes
Savvas

----
Savvas N. Savvides
L-ProBE, Unit for Structural Biology
Ghent University
K.L. Ledeganckstraat 35
9000 Ghent, BELGIUM
Phone: +32-(0)9-264.51.24 ; +32-(0)472-92.85.19
Email: savvas.savvides@ugent.be
http://www.eiwitbiochemie.ugent.be/units_en/structbio_en.html

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Jacob Keller
Sent: Tuesday, November 20, 2007 12:25 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Protein-detergent micelle sizes

Dear Crystallographers,

I am trying to gather literature values on the size of the
detergent/micelle
belts on solublized membrane proteins. Does anybody know of a good
repository of this info, whether as a database or as a review paper, or
otherwise? The rough values of 15-25kD to 35-50kD were given in one
recent
BB posting, but I was looking for a more systematic tabulation...

Thanks,

Jacob Keller

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.467.4049
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Protein-detergent micelle sizes
From: "R {- dot -} M {- dot -} Garavito" garavito {- at -} MSU {- dot -} EDU
Date: 2007-11-20

Next message:
Subject: [SUMMARY]: Twinning problem
From: Andrew Torelli andrew_torelli {- at -} URMC {- dot -} ROCHESTER {- dot -} EDU
Date: 2007-11-20