Re: [ccp4bb] His tag on membrane protein

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: His tag on membrane protein
From: deliang deliangc {- at -} UCI {- dot -} EDU
Date: 2007-11-23

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Subject: Re: Anisotropic data - How to deal with ?
From: Jorge Iulek iulek {- at -} INTERPONTA {- dot -} COM {- dot -} BR
Date: 2007-11-23

Subject: Re: His tag on membrane protein
From: Daniel Picot Daniel {- dot -} Picot {- at -} IBPC {- dot -} FR
Date: 2007-11-23

Hi Deliang,
We have crystallized the complex cytochrome b6f with an extension of
His6 (no linker) at the c-terminal of cytochrome f (Stroebel, 2003). The
His extension takes part to the crystal contacts. The electron density
is visible but not very well defined. We have not tried to remove the
His6. Many other structure of membrane protein have used His-tag, but
still not enough to make sound statistics.
Daniel

deliang a écrit :
> Hi there,
>
> I purified a membrane protein with traditional His-tag on the C
> terminal. Before crystallization, I wonder how this tag may affect the
> result. Does anyone have experience that the removel of this tag may
> improve the result or not? or can provide some references which may give
> some statistic, like how many membrane proteins have been crystallized
> with or without His-tag?
>
> Thanks so much.
>
> Deliang

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: His tag on membrane protein
From: deliang deliangc {- at -} UCI {- dot -} EDU
Date: 2007-11-23

Next message:
Subject: Re: Anisotropic data - How to deal with ?
From: Jorge Iulek iulek {- at -} INTERPONTA {- dot -} COM {- dot -} BR
Date: 2007-11-23