Re: [ccp4bb] To bathe or not to bathe.

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

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   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: To bathe or not to bathe.
From: Richard Gillilan reg8 {- at -} CORNELL {- dot -} EDU
Date: 2007-11-26

Next message:
Subject: Re: To bathe or not to bathe.
From: "Nave, C (Colin)" colin {- dot -} nave {- at -} DIAMOND {- dot -} AC {- dot -} UK
Date: 2007-11-26

Subject: Re: To bathe or not to bathe.
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-11-26

Richard Gillilan wrote:

>
>
> I am currently working on guidelines for when helium and microbeam
> are necessary (based on both simulations and explicit measurements).
> At the present time, my feeling is that crystals below 50 micron can
> certainly make the extra hassle worthwhile. It really depends upon
> how badly you want that extra resolution. In the case above, it
> pushed the resolution from above to below the 2.0 Angstrom mark based
> on I/SIG.
>
Hi Richard,

I/SigI based on which program ? Default modes or tweaked by expert ? I
would give this particular dataset a chance to be processed by all
available programs and then do the comparison, or actualy all the
datasets you have with various setups. Should be a nice table comparing
program X versus Y and Z with the given data. And if it's SeMet data
that would even be better - runnig e.g. Shelx and demonstrating which
setup leads to an interpretable electron density.

Juergen

--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: To bathe or not to bathe.
From: Richard Gillilan reg8 {- at -} CORNELL {- dot -} EDU
Date: 2007-11-26

Next message:
Subject: Re: To bathe or not to bathe.
From: "Nave, C (Colin)" colin {- dot -} nave {- at -} DIAMOND {- dot -} AC {- dot -} UK
Date: 2007-11-26