Re: [ccp4bb] To bathe or not to bathe.

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2007-11-26

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From: Bernhard Rupp bernhardrupp {- at -} SBCGLOBAL {- dot -} NET
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Subject: Re: To bathe or not to bathe.
From: bas {- at -} FREESURF {- dot -} FR bas {- at -} FREESURF {- dot -} FR
Date: 2007-11-26

----- Original Message -----
From: "Nave, C (Colin)"

> Several have raised the issue of radiation damage. The strategy which
> Bob mentions can make sense, ensuring fresh parts of the crystal are
> regularly brought in to the beam. I would have thought 5-10 micron beams
> were rather too small if your crystal is several times bigger than this.
> I think the microbeams can overcook the samples, particularly when
> collecting large rotation ranges. The centre part of the crystal (if
> centred!) is constantly in the beam in this case.

On the other hand, why perfectly centre the crystal? A set-up where a
narrow beam runs past rather than through the rotation axis will spare the
central voxel entirely; apart from a somewhat more often exposed doughnut
around the axis, only fresh parts of the crystal are illuminated that way.

Sebastiaan Werten.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Scientist / Programmer position available
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2007-11-26

Next message:
Subject: Genome Biology 2007 8:103
From: Bernhard Rupp bernhardrupp {- at -} SBCGLOBAL {- dot -} NET
Date: 2007-11-26