[ccp4bb] Easy proteins to crystallize -- summary

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: antibumping restraint in Refmac
From: Serge Cohen s {- dot -} cohen {- at -} NKI {- dot -} NL
Date: 2007-03-21

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Subject: Highest shell standards
From: Shane Atwell satwell {- at -} SGXPHARMA {- dot -} COM
Date: 2007-03-21

Subject: Easy proteins to crystallize -- summary
From: "Douglas L {- dot -} Theobald" dtheobald {- at -} BRANDEIS {- dot -} EDU
Date: 2007-03-21

Thanks all who answered, and sorry for the delay -- I was held up
having a date with a lovely quasi-species of norovirus.

I had asked the list for examples of proteins that are easily
crystallizable, especially those that over-express and diffract well,
not necessarily well-known.

The classics appear to be:

lysozyme
glucose isomerase
thaumatin
alpha-lactalbumin
catalase
ribonuclease S
proteinase K
trypsin
myoglobin
ferritin

For recipes see the Hampton catalog or

http://www.rigaku.com/protein/crystallization.html

Some perhaps lesser known proteins, but nice:

Concanavalin A
CheY
RecA
RluD, an E. coli pseudouridine synthase
Fc portion of antibody
bacterial antitoxin HigA, 2ICT
glycerol dehydrogenase from the yeast S. pombe
tRNA guanine transglycosylase (TGT, e. g. 1n2v)
cytochrome C peroxidase (CCP, e.g. 1AC4)
Cholesterol oxidase from Streptomyces
Beta-lactoglobulin
pepsin
PepN from E.coli

Thanks again!

Douglas

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: antibumping restraint in Refmac
From: Serge Cohen s {- dot -} cohen {- at -} NKI {- dot -} NL
Date: 2007-03-21

Next message:
Subject: Highest shell standards
From: Shane Atwell satwell {- at -} SGXPHARMA {- dot -} COM
Date: 2007-03-21