[ccp4bb] how to change a membrane protein into a water soluble protein?

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CCP4bb <-- 2007 <-- December 2007 <-- 03 December 2007

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Subject: FW: protein induced precipitation of proteins?
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2007-12-03

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Subject: Re: how to change a membrane protein into a water soluble protein?
From: Thomas J Magliery PhD magliery {- at -} CHEMISTRY {- dot -} OHIO-STATE {- dot -} EDU
Date: 2007-12-03

Subject: how to change a membrane protein into a water soluble protein?
From: Daniel Jin jin_xiechen {- at -} YAHOO {- dot -} COM
Date: 2007-12-03

Hi,

I am wondering whether there is a way to turn a membrane protein with known crystal structure into a water soluble protein by systematic mutagenesis. I guess it should be doable if we introduce enough hydrophilic residues on the surface. Has anyone tested this crazy idea before? Thank you for your help.

Best,
Chen

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CCP4bb navigation

CCP4bb <-- 2007 <-- December 2007 <-- 03 December 2007

Previous message:
Subject: FW: protein induced precipitation of proteins?
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2007-12-03