Re: [ccp4bb] how to change a membrane protein into a water soluble protein?

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CCP4bb <-- 2007 <-- December 2007 <-- 03 December 2007

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Subject: how to change a membrane protein into a water soluble protein?
From: Daniel Jin jin_xiechen {- at -} YAHOO {- dot -} COM
Date: 2007-12-03

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Subject: Re: how to change a membrane protein into a water soluble protein?
From: Bil Clemons clemons {- at -} CALTECH {- dot -} EDU
Date: 2007-12-03

Subject: Re: how to change a membrane protein into a water soluble protein?
From: Thomas J Magliery PhD magliery {- at -} CHEMISTRY {- dot -} OHIO-STATE {- dot -} EDU
Date: 2007-12-03

It's hard. See:

J Mol Biol. 2005 May 6;348(3):777-87.
X-ray structure of a water-soluble analog of the membrane protein phospholamban:
sequence determinants defining the topology of tetrameric and pentameric coiled
coils.
Slovic AM, Stayrook SE, North B, Degrado WF.

Slovic, A. M., Summa, C. M., Lear, J. D. & DeGrado,
W. F. (2002). Computational design of a water-soluble
analog of phospholamban. Protein Sci. 12, 337–348.

Li, H., Cocco, M. J., Steitz, T. A. & Engelman, D. E.
(2001). Conversion of phospholamban into a soluble
pentameric helical bundle. Biochemistry, 40,
6636–6645.

Frank, S., Kammerer, R. A., Hellstern, S., Pegoraro, S.,
Stetefeld, J., Lustig, A. et al. (2000). Toward a high resolution
structure of phospholamban: design of
soluble transmembrane domain mutants.
Biochemistry, 39, 6825–6831.

Tom

Daniel Jin wrote:
> Hi,
> I am wondering whether there is a way to turn a membrane protein with
> known crystal structure into a water soluble protein by systematic
> mutagenesis. I guess it should be doable if we introduce enough
> hydrophilic residues on the surface. Has anyone tested this crazy idea
> before? Thank you for your help.
> Best,
> Chen
>
> ------------------------------------------------------------------------
> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try
> it now.
>

--
Thomas J. Magliery, Ph.D.
Assistant Professor
Department of Chemistry
& Department of Biochemistry
The Ohio State University
1043 Evans Laboratory
100 West 18th Ave.
Columbus, OH 43210-1185

(614) 247-8425 office
(614) 292-1685 fax
magliery@chemistry.ohio-state.edu
http://www.chemistry.ohio-state.edu/~magliery

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CCP4bb <-- 2007 <-- December 2007 <-- 03 December 2007

Previous message:
Subject: how to change a membrane protein into a water soluble protein?
From: Daniel Jin jin_xiechen {- at -} YAHOO {- dot -} COM
Date: 2007-12-03

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Subject: Re: how to change a membrane protein into a water soluble protein?
From: Bil Clemons clemons {- at -} CALTECH {- dot -} EDU
Date: 2007-12-03