Re: [ccp4bb] how to change a membrane protein into a water soluble protein?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: how to change a membrane protein into a water soluble protein?
From: Thomas J Magliery PhD magliery {- at -} CHEMISTRY {- dot -} OHIO-STATE {- dot -} EDU
Date: 2007-12-03

Next message:
Subject: ciftr troubles
From: Jan Abendroth jan {- dot -} abendroth {- at -} GMAIL {- dot -} COM
Date: 2007-12-03

Subject: Re: how to change a membrane protein into a water soluble protein?
From: Bil Clemons clemons {- at -} CALTECH {- dot -} EDU
Date: 2007-12-03

There is also the soluble KcsA.

Computational design of water-soluble analogues of the potassium channel
KcsA. A. M. Slovic, H. Kono, J. D. Lear, J. G. Saven, and W. F. DeGrado
(2004) PNAS 101, 1828-1833

Bil

************************************
Bil Clemons, PhD
Assistant Professor of Biochemistry
Caltech
157 Broad Center
MC 114-96
Pasadena, CA 91125
(626) 395-1796
clemons@caltech.edu
************************************

> From: Thomas J Magliery PhD
> Reply-To: Thomas J Magliery PhD
> Date: Mon, 3 Dec 2007 16:50:03 -0500
> To:
> Subject: Re: [ccp4bb] how to change a membrane protein into a water soluble
> protein?
>
> It's hard. See:
>
> J Mol Biol. 2005 May 6;348(3):777-87.
> X-ray structure of a water-soluble analog of the membrane protein
> phospholamban:
> sequence determinants defining the topology of tetrameric and pentameric
> coiled
> coils.
> Slovic AM, Stayrook SE, North B, Degrado WF.
>
> Slovic, A. M., Summa, C. M., Lear, J. D. & DeGrado,
> W. F. (2002). Computational design of a water-soluble
> analog of phospholamban. Protein Sci. 12, 337348.
>
> Li, H., Cocco, M. J., Steitz, T. A. & Engelman, D. E.
> (2001). Conversion of phospholamban into a soluble
> pentameric helical bundle. Biochemistry, 40,
> 66366645.
>
> Frank, S., Kammerer, R. A., Hellstern, S., Pegoraro, S.,
> Stetefeld, J., Lustig, A. et al. (2000). Toward a high resolution
> structure of phospholamban: design of
> soluble transmembrane domain mutants.
> Biochemistry, 39, 68256831.
>
> Tom
>
>
> Daniel Jin wrote:
>> Hi,
>> I am wondering whether there is a way to turn a membrane protein with
>> known crystal structure into a water soluble protein by systematic
>> mutagenesis. I guess it should be doable if we introduce enough
>> hydrophilic residues on the surface. Has anyone tested this crazy idea
>> before? Thank you for your help.
>> Best,
>> Chen
>>
>> ------------------------------------------------------------------------
>> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try
>> it now.
>>
>> DtDypao8Wcj9tAcJ%20>
>
>
> --
> Thomas J. Magliery, Ph.D.
> Assistant Professor
> Department of Chemistry
> & Department of Biochemistry
> The Ohio State University
> 1043 Evans Laboratory
> 100 West 18th Ave.
> Columbus, OH 43210-1185
>
> (614) 247-8425 office
> (614) 292-1685 fax
> magliery@chemistry.ohio-state.edu
> http://www.chemistry.ohio-state.edu/~magliery
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: ciftr troubles
From: Jan Abendroth jan {- dot -} abendroth {- at -} GMAIL {- dot -} COM
Date: 2007-12-03