Re: [ccp4bb] PEG MW vs. cryoprotectivity

- Protein crystallography

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- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: SeMet peptide synthesis
From: Torres-Larios Alfredo torres {- at -} IFC {- dot -} UNAM {- dot -} MX
Date: 2007-12-05

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From: Martyn Winn m {- dot -} d {- dot -} winn {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-12-06

Subject: Re: PEG MW vs. cryoprotectivity
From: Jennifer Cash Jncash44 {- at -} AOL {- dot -} COM
Date: 2007-12-05

In our recent experience, larger PEGs work similarly to smaller PEGs as far
as vitrification goes. Additionally, transferring crystals to a solution of
increased PEG concentration (as compared to mother liquor) can substantially
reduce the amount of cryo needed for freezing and can be more gentle on crystals
than just using a high concentration of cryo. Here is a reference that
tests cryoprotective ability of PEG 2000 & 20000.
J. Appl. Cryst. (2006) 39, 244-251

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: SeMet peptide synthesis
From: Torres-Larios Alfredo torres {- at -} IFC {- dot -} UNAM {- dot -} MX
Date: 2007-12-05

Next message:
Subject: BIOXHIT/BCO protein crystallography course in Oulu, Finland
From: Martyn Winn m {- dot -} d {- dot -} winn {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-12-06