Quick navigation:        Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |   
Protein structure
 

[ccp4bb] Purification using GST column at low pH
 

Basic tutorials: 		 
 

CCP4bb navigation

CCP4bb <-- 2007 <-- December 2007 <-- 07 December 2007
Previous message:
Subject: heavy atom source summary
From: "Joyce, Gordon M {- dot -} (NIH/NIAID) [F]" joycego {- at -} NIAID {- dot -} NIH {- dot -} GOV
Date: 2007-12-07 		Next message:
Subject: Re: SUMMARY: PEG MW vs. cryoprotectivity
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-12-07


Subject: Purification using GST column at low pH
From: E rajakumar e_rajakumar {- at -} YAHOO {- dot -} COM
Date: 2007-12-07
Dear All
Sorry for non-crystallography question. I am purifying
a protein using GST column. The gel filtration
chromatography is indicating that the protein is
aggregating at pH more than 7.0. I am planning to
purify the protein at the pH around 6.0. So, I want to
know the affinity of GST-fusion protein to the GST
column and also activity of the Prescission Protease
at pH 6.0.
Thank you in advance
Rajakumara




Send instant messages to your online friends http://uk.messenger.yahoo.com

CCP4bb navigation

CCP4bb <-- 2007 <-- December 2007 <-- 07 December 2007
Previous message:
Subject: heavy atom source summary
From: "Joyce, Gordon M {- dot -} (NIH/NIAID) [F]" joycego {- at -} NIAID {- dot -} NIH {- dot -} GOV
Date: 2007-12-07 		Next message:
Subject: Re: SUMMARY: PEG MW vs. cryoprotectivity
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-12-07


ProteinCrystallography.org: Copyright 2006-2008 by Quid United Ltd