Re: [ccp4bb] Scale factor in ccp4

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: iMosflm 0.5.2 available now
From: Harry Powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-03-22

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Subject: Re: Scale factor in ccp4
From: Johan Turkenburg jpt {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-22

Subject: Re: Scale factor in ccp4
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-22

When youu run scalepack2mtz the GUI always follows this by TRUNCATE to
convert Is to Fs

At that stage there is an attempt to put the data on roughly an absolute
scale, either using the NRES you gave as input or if that is not set, I
think assuming 50% of the cell volume is protein.
Anyway the scales WILL be different after TRUNCATE.

If you want to scale them together more carefully you will need to run
cad, then SCALEIT ( on the GUI undr exptl phases)
THEN convert each I1 and I2 back to a sca file ..

Seems a lot of trouble! Why do you need this?

Eleanor

yang li wrote:
> Hi:
> I have two set of data from the same crystal with the names 1.sca
> and 2.sca,
> they have different Intensity values due to different scale factors.
> Now I use Scalepack2mtz
> convert them to 1.mtz and 2.mtz, then use cad to merge to a cad.mtz, then
> convert it to cad.sca file, I find that the Intensity values in this
> cad.sca arediffrent from
> 1.sca and 2.sca, I wonder if the program has scaled the values itself?
> If that is true,
> which program did this, Scalepack2mtz or cad?
> Thanks!
>
> Li Yang
>
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: iMosflm 0.5.2 available now
From: Harry Powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-03-22

Next message:
Subject: Re: Scale factor in ccp4
From: Johan Turkenburg jpt {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-22