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Re: [ccp4bb] detecting RNA from possible protein-RNA complex crystals
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: HP OS X10.5 advice needed
From: james {- dot -} phillips {- at -} DUKE {- dot -} EDU james {- dot -} phillips {- at -} DUKE {- dot -} EDU
Date: 2007-12-12		Next message:
Subject: Re: detecting RNA from possible protein-RNA complex crystals
From: Kevin Jude kjude {- at -} BERKELEY {- dot -} EDU
Date: 2007-12-12


Subject: Re: detecting RNA from possible protein-RNA complex crystals
From: Alan Cheung cheung {- at -} LMB {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2007-12-12

If you have a fluorescence microscope, you could try soaking SYBR-Gold
dye into your crystals.

Kettenberger, H., and Cramer, P. (2006). Fluorescence detection of
nucleic acids and proteins in multi-component crystals. Acta Crystallogr
D Biol Crystallogr 62, 146-150.

Alan



Rongjin Guan wrote:
> Dear All
>
> I am trying to co-crystallize a protein and dsRNA complex, and looking
> for methods
> to detect RNA in the crystals. I am thinking about using Mass Spectrum,
> Nanodrop to
> measure OD280/260, etc, but I wonder if ther are some more sensitive and
> reliable ways.
>
> The crystals are grown in high concentration salts; the size is usually
> small but I do
> have several big ones which can be easily fished out. They can be dyed
> so definitely
> not salt crystals.
>
> Thanks
>
> Rongjin Guan

--
Alan Cheung
Gene Center
Ludwig-Maximilians-University
Feodor-Lynen-Str. 25
81377 Munich
Germany
Phone: +49-89-2180-76845
Fax: +49-89-2180-76999
E-mail: cheung@lmb.uni-muenchen.de

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: HP OS X10.5 advice needed
From: james {- dot -} phillips {- at -} DUKE {- dot -} EDU james {- dot -} phillips {- at -} DUKE {- dot -} EDU
Date: 2007-12-12		Next message:
Subject: Re: detecting RNA from possible protein-RNA complex crystals
From: Kevin Jude kjude {- at -} BERKELEY {- dot -} EDU
Date: 2007-12-12
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