Re: [ccp4bb] detecting RNA from possible protein-RNA complex crystals

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: detecting RNA from possible protein-RNA complex crystals
From: Alan Cheung cheung {- at -} LMB {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2007-12-12

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Subject: Re: detecting RNA from possible protein-RNA complex crystals
From: Kevin Jude kjude {- at -} BERKELEY {- dot -} EDU
Date: 2007-12-12

You could stain your crystals directly with Sybr Gold, which will
fluoresce upon binding nucleic acids, or you can visualize both protein
and RNA on a silver-stained SDS-PAGE gel.

Kevin

Rongjin Guan wrote:
> Dear All
>
> I am trying to co-crystallize a protein and dsRNA complex, and
> looking for methods to detect RNA in the crystals. I am thinking
> about using Mass Spectrum, Nanodrop to measure OD280/260, etc, but I
> wonder if ther are some more sensitive and reliable ways.
>
> The crystals are grown in high concentration salts; the size is
> usually small but I do have several big ones which can be easily
> fished out. They can be dyed so definitely not salt crystals.
>
> Thanks
>
> Rongjin Guan

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: detecting RNA from possible protein-RNA complex crystals
From: Alan Cheung cheung {- at -} LMB {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2007-12-12

Next message:
Subject: Rigaku crystallography system - negotiable price
From: Erin Curry erin {- at -} AAISOLUTIONS {- dot -} COM
Date: 2007-12-12