Re: [ccp4bb] artificial dimerization modules

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: artificial dimerization modules
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-12-18

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Subject: Re: Fwd: Occupancy refinement of substrate using refmac5
From: Zheng Zhou zhengzhouzz {- at -} GMAIL {- dot -} COM
Date: 2007-12-18

Subject: Re: artificial dimerization modules
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-12-18

C-terminal 'clasp' domain of any bacterial gyrA or parC - very small,
pretty tight dimers.

Artem

> This is not strictly a crystallography question, but I imagine (and
> hope) that some of you would be able to advise me. In short, I would
> like to add to t7he N-terminal part of my construct a sequence that will
> promote dimerization of the molecule.
>
> Background: I am working on a large protein that is built from multiple
> domains. I am interested in the structure of some of the more C-terminal
> domains. Obviously, I do not have well diffracting crystals at this
> point (and hence this post). N-terminal to the domains of interest is a
> dimerization domain. I would like to mimic the dimerization induced by
> this domain by adding as short as possible a sequence. Moreover, I would
> like to try both a parallel and an anti-parallel dimerization motif.
>
> Would you know of an appropriate short sequence (e.g. one that makes a
> helix which dimerizes), or of a very small domain that I could add to my
> construct?
>
> Thanks.
>
> Arnon
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: artificial dimerization modules
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-12-18

Next message:
Subject: Re: Fwd: Occupancy refinement of substrate using refmac5
From: Zheng Zhou zhengzhouzz {- at -} GMAIL {- dot -} COM
Date: 2007-12-18