[ccp4bb] Twinned crystals to single crystal

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: problems of running mosflm on the MAC
From: michael nelson gnomenm {- at -} YAHOO {- dot -} COM
Date: 2007-12-21

Next message:
Subject: Re: Twinned crystals to single crystal
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2007-12-21

Subject: Twinned crystals to single crystal
From: shivesh kumar 2shivesh {- at -} GMAIL {- dot -} COM
Date: 2007-12-21

Dear all,
I have crystallized a protein in 63% of MPD at pH 3.8-4.6.The crystals are
twinned but with sharp edges.I welcome all the suggestions to get single
crystals.Also,I have needles of other mutant protein,the problem is during
concentration,it gets precipitated even in the centricon.What should I do to
avoid this precipitation to have a concentrated protein.Thanx in advance for
the suggestions.
Shivesh

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: problems of running mosflm on the MAC
From: michael nelson gnomenm {- at -} YAHOO {- dot -} COM
Date: 2007-12-21

Next message:
Subject: Re: Twinned crystals to single crystal
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2007-12-21