[ccp4bb] Plz suggest me

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

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- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Refmac 5.4.0066 versus Refmac 5.4.0034
From: Mark A Saper saper {- at -} UMICH {- dot -} EDU
Date: 2007-12-31

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Subject: Re: Plz suggest me
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-01-01

Subject: Plz suggest me
From: "Md {- dot -} Imtiyaz Hassan" imtaiyazh {- at -} YAHOO {- dot -} CO {- dot -} UK
Date: 2008-01-01

Dear freinds

We have crystallized a cyanobacterial phycoerythrin which having two (alpha subunit) in an assymetric unit and held together by only three hydrogen bonds. When we compare the A and B molecule the RMSD is 1.32. because they same sequences then how it is possible to that two molecules are deviated at such extent. now the question is

are both the molecules are crystallographic dimer?

are two molecules are structural subunit as alpha 1 and alpha2?

Is there any problem in refinement ? The structure was refined at 2.6 A with R 0.23/0.28
I hope a possible suggestion
with warm regards

Have a nice day......

Md. Imtiyaz Hassan, Ph.D.
Young Scientist
CIRBSc, JMI
Telefax: +91 11 26983409
Mob-9868311151

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Refmac 5.4.0066 versus Refmac 5.4.0034
From: Mark A Saper saper {- at -} UMICH {- dot -} EDU
Date: 2007-12-31

Next message:
Subject: Re: Plz suggest me
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-01-01