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Re: [ccp4bb] Plz suggest me

 

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CCP4bb <-- 2008 <-- January 2008 <-- 01 January 2008
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Subject: Plz suggest me
From: "Md {- dot -} Imtiyaz Hassan" imtaiyazh {- at -} YAHOO {- dot -} CO {- dot -} UK
Date: 2008-01-01
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Date: 2008-01-01


Subject: Re: Plz suggest me
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-01-01

could you please be more specific how you refined the structure, what program have you been using and which restraints did you set for the two independent molecules.

Best wishes

Kornelius

On Tue, 1 Jan 2008 11:42:40 +0000
"Md. Imtiyaz Hassan" wrote:
>
>
> Dear freinds
>
> We have crystallized a cyanobacterial phycoerythrin which having two (alpha subunit) in an assymetric unit and held together by only three hydrogen bonds. When we compare the A and B molecule the RMSD is 1.32. because they same sequences then how it is possible to that two molecules are deviated at such extent. now the question is
>
>
>
> are both the molecules are crystallographic dimer?
>
> are two molecules are structural subunit as alpha 1 and alpha2?
>
> Is there any problem in refinement ? The structure was refined at 2.6 A with R 0.23/0.28
> I hope a possible suggestion
> with warm regards
>
> Have a nice day......
>
> Md. Imtiyaz Hassan, Ph.D.
> Young Scientist
> CIRBSc, JMI
> Telefax: +91 11 26983409
> Mob-9868311151
>
>
>
>
>
> __________________________________________________________
> Sent from Yahoo! Mail - a smarter inbox http://uk.mail.yahoo.com

----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
kornelius.zeth@tuebingen.mpg.de
Tel -49 7071 601 323
Fax -49 7071 601 349




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