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Re: [ccp4bb] Crystallographic Experiment with NADH and a Flavoprotein
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
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Subject: Only Five Weeks left to apply for the BNL-Biology and NSLS Course on Synchrotron Data Collection
From: Robert Sweet sweet {- at -} BNL {- dot -} GOV
Date: 2008-01-01		Next message:
Subject: c-terminus modification in crystals
From: siva charan siva {- dot -} charan {- at -} UNI-GRAZ {- dot -} AT
Date: 2008-01-02


Subject: Re: Crystallographic Experiment with NADH and a Flavoprotein
From: John Berrisford jmb {- at -} MRC-DUNN {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-01-02

Juan Sanchez Weatherby wrote:
>
> The fact that you cannot find structures with NADH is because from the
> crystallographic point of view, as you cannot see the hydrogens, then
> both NAD+ and NADH are the same structure and are thus labeled as
> "NAD" in a PDB. Therefore if you are looking for a NADH structure you
> need to look for the structure of a NAD containing enzyme in the
> reduced state.
NADH has the label "NAI" in the pdb, where as NAD+ is labelled "NAD".
NAD+ and NADH are also slightly different at the nicotinamide ring due
to the extra hydrogen.



--
John Berrisford

Medical Research Council
Dunn Human Nutrition Unit
Wellcome Trust / MRC Building
Hills Road
Cambridge
CB2 2XY
WEB: www.mrc-dunn.cam.ac.uk
Tel: +44 1223 252882


CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Only Five Weeks left to apply for the BNL-Biology and NSLS Course on Synchrotron Data Collection
From: Robert Sweet sweet {- at -} BNL {- dot -} GOV
Date: 2008-01-01		Next message:
Subject: c-terminus modification in crystals
From: siva charan siva {- dot -} charan {- at -} UNI-GRAZ {- dot -} AT
Date: 2008-01-02
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