[ccp4bb] c-terminus modification in crystals

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Crystallographic Experiment with NADH and a Flavoprotein
From: John Berrisford jmb {- at -} MRC-DUNN {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-01-02

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From: Joel Janin joel {- dot -} janin {- at -} U-PSUD {- dot -} FR
Date: 2008-01-02

Subject: c-terminus modification in crystals
From: siva charan siva {- dot -} charan {- at -} UNI-GRAZ {- dot -} AT
Date: 2008-01-02

Dear All,

I have solved crystal structure of a protein at 1.8 Å resolution using
molecular replacement. Interestingly I could see extra electron density
at the C-terminus end. The extra electron density is like split going in
to two directions.We have checked with MS on protein in solution for
extra residues but we did not find any extra molecular weight.
C-terminal sequence is verified on the DNA level by DNA
sequencing. Protein took around 30 days to crystallize, it has PEG 3350
as precipitant, around 0.1 M of NaCl and 0.2 M bis-tris at pH 5.5. Does
any body have idea about these crystallizing agents doing c-terminus
modification? I am attaching a snap shot of the extra electron density.
I would be happy to receive information about reference about main chain
modifications in crystallization process.

Kind regards &

Wish you all a happy and successful new year

Siva

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Crystallographic Experiment with NADH and a Flavoprotein
From: John Berrisford jmb {- at -} MRC-DUNN {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-01-02

Next message:
Subject: Call for targets: the CAPRI experiment
From: Joel Janin joel {- dot -} janin {- at -} U-PSUD {- dot -} FR
Date: 2008-01-02