[ccp4bb] resolution & R-free

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Webcast of the CCP4 study weekend ?
From: Martyn Winn m {- dot -} d {- dot -} winn {- at -} DL {- dot -} AC {- dot -} UK
Date: 2008-01-03

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Subject: Neutron Diffraction Instrument Scientist
From: Leighton Coates coatesl {- at -} SNS {- dot -} GOV
Date: 2008-01-03

Subject: resolution & R-free
From: ÑîÁøÇà yangliuqing1984 {- at -} HOTMAIL {- dot -} COM
Date: 2008-01-03

Hi,dear all:
I try to refine one protein structure using refmac5,now I got some problems.
For example,first,the resolution is 2.25,R-free is 30,completeness is 90%.
Then I changed resolution to 2.35,R-free became 28.0,completeness is 91%.
When I changed resolution to 2.45,R-free became 27.5,completeness is 92%.
So,when resolution from 2.25 to 2.35,R-free goes down a lot,but resolution from 2.35 to 2.45,R-free nearly the same,is that meaning resolution should around 2.35? Should I change resolution during refinement? And is there any good software for refinement?
Thanks a lot!
Best wishes!
liuqing

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Webcast of the CCP4 study weekend ?
From: Martyn Winn m {- dot -} d {- dot -} winn {- at -} DL {- dot -} AC {- dot -} UK
Date: 2008-01-03

Next message:
Subject: Neutron Diffraction Instrument Scientist
From: Leighton Coates coatesl {- at -} SNS {- dot -} GOV
Date: 2008-01-03