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[ccp4bb] Protein DNA complex refinement |
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CCP4bb navigationCCP4bb <-- 2008 <-- January 2008 <-- 03 January 2008Subject: Protein DNA complex refinement From: Daouda TRAORE daouda {- dot -} traore {- at -} IBS {- dot -} FR Date: 2008-01-03 I'm trying to refine a protein DNA complex using Refmac. Unfortunately the job fails because of the definition of nucleosides : In the pdb file I use for molecular replacement (and it's the same for all protein-DNA complex I found in the PDB) nucleosides are labelled DG, DT, DA , DC. In the monomer libraries for Refmac these are labelled GD, TD, AD, CD. The jobs fails also if I replace the "DG, DT, DA , DC" by "GD, TD, AD, CD" in the coordinate file prior to refinement. Could someone help me. -- -------------------------------------------------------- Daouda A.K. TRAORE, PhD Student Laboratoire de Cristallographie et Cristallogenèse des Protéines Institut de Biologie Structurale de Grenoble 41 rue Jules Horowitz 38027 Grenoble Cedex 1 - France Phone: @IBS: +33 4 38 78 95 96 @ESRF/FIP: +33 4 76 88 27 89 Fax: +33 4 38 78 51 22 ************************ Laboratoire Chimie et Biologie des Metaux CEA Grenoble iRTSV / LCBM / PMB 17 Avenue des Martyrs 38054 Grenoble Cedex 9 - France Phone : +33 4 38 78 38 75 Fax : +33 4 38 78 34 62 E-mail: daouda.traore@ibs.fr -------------------------------------------------------- CCP4bb navigationCCP4bb <-- 2008 <-- January 2008 <-- 03 January 2008 |
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