[ccp4bb] Protein DNA complex refinement

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: resolution & R-free -- addenda
From: Pavel Afonine pafonine {- at -} LBL {- dot -} GOV
Date: 2008-01-03

Next message:
Subject: Re: Protein DNA complex refinement
From: Garib Murshudov garib {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-01-03

Subject: Protein DNA complex refinement
From: Daouda TRAORE daouda {- dot -} traore {- at -} IBS {- dot -} FR
Date: 2008-01-03

Hi all,
I'm trying to refine a protein DNA complex using Refmac.
Unfortunately the job fails because of the definition of nucleosides :
In the pdb file I use for molecular replacement (and it's the same for
all protein-DNA complex I found in the PDB) nucleosides are labelled DG,
DT, DA , DC. In the monomer libraries for Refmac these are labelled GD,
TD, AD, CD.
The jobs fails also if I replace the "DG, DT, DA , DC" by "GD, TD, AD,
CD" in the coordinate file prior to refinement.
Could someone help me.

--
--------------------------------------------------------
Daouda A.K. TRAORE, PhD Student

Laboratoire de Cristallographie et Cristallogenèse des Protéines
Institut de Biologie Structurale de Grenoble
41 rue Jules Horowitz
38027 Grenoble Cedex 1 - France
Phone: @IBS: +33 4 38 78 95 96 @ESRF/FIP: +33 4 76 88 27 89
Fax: +33 4 38 78 51 22

************************

Laboratoire Chimie et Biologie des Metaux
CEA Grenoble iRTSV / LCBM / PMB
17 Avenue des Martyrs
38054 Grenoble Cedex 9 - France
Phone : +33 4 38 78 38 75
Fax : +33 4 38 78 34 62

E-mail: daouda.traore@ibs.fr
--------------------------------------------------------

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: resolution & R-free -- addenda
From: Pavel Afonine pafonine {- at -} LBL {- dot -} GOV
Date: 2008-01-03

Next message:
Subject: Re: Protein DNA complex refinement
From: Garib Murshudov garib {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-01-03